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PGL4.11-COL1A1mSMAD
(Plasmid #230917)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 230917 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PGL4.11
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4370
  • Total vector size (bp) 5350
  • Vector type
    Mammalian Expression, Bacterial Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Human COL1A1 promoter with mutant SMAD binding sequence
  • Alt name
    PGL4.11-COLA1mSMAD
  • Species
    H. sapiens (human)
  • Mutation
    COL1A1 promoter with mutant potential SMAD binding sides from -(611) to -(608)
  • GenBank ID
    NC_000017.11 (50184096..50201649), complement)
  • Entrez Gene
    COL1A1 (a.k.a. CAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4)
  • Promoter COL1A1
  • Tag / Fusion Protein
    • Luciferase-luc2p (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ACC65I (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer RVprimer3
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PGL4.11-COL1A1mSMAD was a gift from Avi Rosenberg (Addgene plasmid # 230917 ; http://n2t.net/addgene:230917 ; RRID:Addgene_230917)

  • For your References section:

    Bile Acid Receptor Agonist Reverses Transforming Growth Factor-beta1-Mediated Fibrogenesis in Human Induced Pluripotent Stem Cells-Derived Kidney Organoids. Yang X, Delsante M, Daneshpajouhnejad P, Fenaroli P, Mandell KP, Wang X, Takahashi S, Halushka MK, Kopp JB, Levi M, Rosenberg AZ. Lab Invest. 2024 May;104(5):100336. doi: 10.1016/j.labinv.2024.100336. Epub 2024 Jan 22. 10.1016/j.labinv.2024.100336 PubMed 38266922
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