PGL4.11-COL1A1mTEAD
(Plasmid #230916)

• Purpose: Comparing with wild type COL1A1 promoter, tested whether mutant TEAD binding sites changed the promoter activity.
• Depositing Lab: Avi Rosenberg
• Publication: Yang et al Lab Invest. 2024 May;104(5):100336. doi: 10.1016/j.labinv.2024.100336. Epub 2024 Jan 22.

Backbone

• Vector backbone: PGL4.11
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4370
• Total vector size (bp): 5350
• Vector type: Mammalian Expression, Bacterial Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Human COL1A1 promoter with mutant TEAD elements
• Alt name: PGL4.11-COLA1mTEAD
• Species: H. sapiens (human)
• Insert Size (bp): 980
• Mutation: mutant potential TEAD bind elements AGGAAT to CTGCGT from -(600) to -(604)
• GenBank ID: NC_000017.11
• Entrez Gene: COL1A1 (a.k.a. CAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4)
• Promoter: COL1A1
• Tag / Fusion Protein:
  • Luciferase- luc2p (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ACC65I (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: RVprimer3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry