PGL4.11-COL1A1WT
(Plasmid
#230915)
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PurposeExpression of human COL1A1 promoter and testing the promoter activity with luciferase assay.
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Depositing Lab
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Depositing OrganizationJohns Hopkins University and School of Medicine
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 230915 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 230915-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 230915-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonePGL4.11
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4370
- Total vector size (bp) 5350
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Vector typeMammalian Expression, Bacterial Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHuman COL1A1 promoter
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SpeciesH. sapiens (human)
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Insert Size (bp)980
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GenBank IDNC_000017.11 (50184096..50201649), complement)
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Entrez GeneCOL1A1 (a.k.a. CAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4)
- Promoter COL1A1
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Tag
/ Fusion Protein
- luciferase - Luc2p (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ACC65I (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer RVprimer3
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 230915-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 230915-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PGL4.11-COL1A1WT was a gift from Avi Rosenberg (Addgene plasmid # 230915 ; http://n2t.net/addgene:230915 ; RRID:Addgene_230915) -
For your References section:
Bile Acid Receptor Agonist Reverses Transforming Growth Factor-beta1-Mediated Fibrogenesis in Human Induced Pluripotent Stem Cells-Derived Kidney Organoids. Yang X, Delsante M, Daneshpajouhnejad P, Fenaroli P, Mandell KP, Wang X, Takahashi S, Halushka MK, Kopp JB, Levi M, Rosenberg AZ. Lab Invest. 2024 May;104(5):100336. doi: 10.1016/j.labinv.2024.100336. Epub 2024 Jan 22. 10.1016/j.labinv.2024.100336 PubMed 38266922
