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Addgene

UAST-uMCas12a+nls2x
(Plasmid #230909)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 230909 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUAST
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas12a+

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.11.26.625385 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UAST-uMCas12a+nls2x was a gift from Michael Boutros (Addgene plasmid # 230909 ; http://n2t.net/addgene:230909 ; RRID:Addgene_230909)

  • For your References section:

    Enhanced in vivo gene knockout with undetectable off-targets using multiplexed Cas12a sgRNAs. Port F, Buhmann MA, Zhou J, Stricker M, Vaughan-Brown A, Michalsen A-C, Roßmanith E, Pöltl A, Großkurth L, Huber J, Menendez Kury LB, Weberbauer B, Hübl M, Heigwer F, Boutros M. bioRxiv 2024.11.26.625385 10.1101/2024.11.26.625385
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