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pTUM4
(Plasmid #230905)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 230905 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACYC184
  • Total vector size (bp) 5914
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    FkpA peptidyl-prolyl cis-trans isomerase FkpA
  • Alt name
    947850
  • Species
    E. coli
  • Insert Size (bp)
    810
  • GenBank ID
    NP_417806
  • Entrez Gene
    fkpA (a.k.a. b3347, ECK3334, yzzS)

Gene/Insert 2

  • Gene/Insert name
    SurA
  • Alt name
    944812
  • Species
    E. coli
  • Insert Size (bp)
    1284
  • GenBank ID
    NP_414595
  • Entrez Gene
    surA (a.k.a. b0053, ECK0054)

Gene/Insert 3

  • Gene/Insert name
    DsbA
  • Alt name
    948353
  • Species
    E. coli
  • Insert Size (bp)
    624
  • GenBank ID
    NP_418297
  • Entrez Gene
    dsbA (a.k.a. b3860, ECK3852, dsf, iarA, ppfA)

Gene/Insert 4

  • Gene/Insert name
    DsbC
  • Alt name
    947363
  • Species
    E. coli
  • Insert Size (bp)
    708
  • GenBank ID
    NP_417369
  • Entrez Gene
    dsbC (a.k.a. b2893, ECK2888, xprA)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTUM4 was a gift from Arne Skerra (Addgene plasmid # 230905 ; http://n2t.net/addgene:230905 ; RRID:Addgene_230905)

  • For your References section:

    A system for concomitant overexpression of four periplasmic folding catalysts to improve secretory protein production in Escherichia coli. Schlapschy M, Grimm S, Skerra A. Protein Eng Des Sel. 2006 Aug;19(8):385-90. doi: 10.1093/protein/gzl018. Epub 2006 May 23. 10.1093/protein/gzl018 PubMed 16720693
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