pEt28a-SilkTag-CBM-H6
(Plasmid
#230903)
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PurposeBacterial expression of SilkTag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 230903 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEt28a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSilkTag-CBM-H6
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Tag
/ Fusion Protein
- HisTag
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEt28a-SilkTag-CBM-H6 was a gift from Sesilja Aranko (Addgene plasmid # 230903 ; http://n2t.net/addgene:230903 ; RRID:Addgene_230903) -
For your References section:
Biomolecular Click Reactions Using a Minimal pH-Activated Catcher/Tag Pair for Producing Native-Sized Spider-Silk Proteins. Fan R, Hakanpaa J, Elfving K, Taberman H, Linder MB, Aranko AS. Angew Chem Int Ed Engl. 2023 Mar 6;62(11):e202216371. doi: 10.1002/anie.202216371. Epub 2023 Feb 7. 10.1002/anie.202216371 PubMed 36695475