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Addgene

pEt28a-H6-SUMO-SilkCatcher
(Plasmid #230902)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 230902 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEt28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    H6-SUMO-SilkCatcher
  • Tag / Fusion Protein
    • HisTag, thrombin, SUMO

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEt28a-H6-SUMO-SilkCatcher was a gift from Sesilja Aranko (Addgene plasmid # 230902 ; http://n2t.net/addgene:230902 ; RRID:Addgene_230902)

  • For your References section:

    Biomolecular Click Reactions Using a Minimal pH-Activated Catcher/Tag Pair for Producing Native-Sized Spider-Silk Proteins. Fan R, Hakanpaa J, Elfving K, Taberman H, Linder MB, Aranko AS. Angew Chem Int Ed Engl. 2023 Mar 6;62(11):e202216371. doi: 10.1002/anie.202216371. Epub 2023 Feb 7. 10.1002/anie.202216371 PubMed 36695475
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