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Addgene

pGH394
(Plasmid #230851)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 230851 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGH125
  • Modifications to backbone
    Inserted PmCDA1 instead of dCas9
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rAPOBEC1-dCas9

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.11.18.621003 for the bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGH394 was a gift from Gaelen Hess (Addgene plasmid # 230851 ; http://n2t.net/addgene:230851 ; RRID:Addgene_230851)

  • For your References section:

    Towards optimizing diversifying base editors for high-throughput studies of single- nucleotide variants. Schwartz CI, Abell NS, Li A, Aradhana, Tycko J, Truong A, Montgomery SB, Hess GT. bioRxiv [Preprint]. 2024 Nov 19:2024.11.18.621003. doi: 10.1101/2024.11.18.621003. 10.1101/2024.11.18.621003 PubMed 39605325
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