pDEST15-hOPTN-D474N
(Plasmid
#23054)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 23054 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDEST15
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5300
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameoptineurin
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SpeciesH. sapiens (human)
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Insert Size (bp)1733
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MutationD474N
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Entrez GeneOPTN (a.k.a. ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attR (unknown if destroyed)
- 3′ cloning site attR (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST15-hOPTN-D474N was a gift from Jon Ashwell (Addgene plasmid # 23054 ; http://n2t.net/addgene:23054 ; RRID:Addgene_23054) -
For your References section:
Optineurin negatively regulates TNFalpha- induced NF-kappaB activation by competing with NEMO for ubiquitinated RIP. Zhu G, Wu CJ, Zhao Y, Ashwell JD. Curr Biol. 2007 Aug 21. 17(16):1438-43. 10.1016/j.cub.2007.07.041 PubMed 17702576