pDEST26-hOPTN-D474N
(Plasmid #23051)

• Depositing Lab: Jon Ashwell
• Publication: Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43.

Backbone

• Vector backbone: pDEST26
• Backbone size w/o insert (bp): 5800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Optineurin
• Species: H. sapiens (human)
• Insert Size (bp): 1733
• Mutation: D474N
• Entrez Gene: OPTN (a.k.a. ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP)
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attR (unknown if destroyed)
• 3′ cloning site: attR (unknown if destroyed)
• 5′ sequencing primer: CMVPro Fwd

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry