T7-p73DD L371P-pcDNA3
(Plasmid #23049)

• Depositing Lab: William Kaelin
• Publication: Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5484
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tumor protein p73
• Alt name: TP73, isoform a
• Species: H. sapiens (human)
• Insert Size (bp): 942
• Mutation: truncated cDNA encodes truncated protein (aa 327-636) with dominant negative function, L371P: point mutation at codon 371 changing from Leucin to Proline ablating the dominant negative function
• GenBank ID: NM_005427
• Entrez Gene: TP73 (a.k.a. CILD47, P73)
• Tag / Fusion Protein:
  • T7 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH I (not destroyed)
• 3′ cloning site: EcoR I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry