pDUP 4-5
(Plasmid
#23023)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 23023 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneDUP33
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Backbone manufacturerZ Dominski and R Kole
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namec-src
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Alt namesrc
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SpeciesM. musculus (mouse)
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Entrez GeneSrc (a.k.a. pp60c-src)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NsiI (not destroyed)
- 3′ cloning site DraIII (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
DUP4-5 (Fig. 2A) was made by digesting a src minigene construct (Fig. 1A)
with ApaI and BamHI and inserting the fragment containing the N1 exon into
DUP4-1 (Addgene Plasmid 23022) at the ApaI and BglII sites.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDUP 4-5 was a gift from Douglas Black (Addgene plasmid # 23023 ; http://n2t.net/addgene:23023 ; RRID:Addgene_23023) -
For your References section:
A complex intronic splicing enhancer from the c-src pre-mRNA activates inclusion of a heterologous exon. Modafferi EF, Black DL. Mol Cell Biol. 1997 Nov . 17(11):6537-45. PubMed 9343417
Map uploaded by the depositor.
