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Addgene

pET15b-hnRNP-F
(Plasmid #23021)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 23021 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hnRNP-F
  • Alt name
    hnRNPF
  • Alt name
    HNRPF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Entrez Gene
    HNRNPF (a.k.a. HNRPF, OK/SW-cl.23, mcs94-1)
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b-hnRNP-F was a gift from Douglas Black (Addgene plasmid # 23021 ; http://n2t.net/addgene:23021 ; RRID:Addgene_23021)

  • For your References section:

    hnRNP H is a component of a splicing enhancer complex that activates a c-src alternative exon in neuronal cells. Chou MY, Rooke N, Turck CW, Black DL. Mol Cell Biol. 1999 Jan . 19(1):69-77. PubMed 9858532
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