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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 23020 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET15b
- Backbone size w/o insert (bp) 5700
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehnRNP-H
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Alt namehnRNPH
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Alt nameHNRPH
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SpeciesH. sapiens (human)
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Insert Size (bp)1300
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Entrez GeneHNRNPH1 (a.k.a. HNRPH, HNRPH1, NEDCDS, hnRNPH)
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Tag
/ Fusion Protein
- His (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the hnRNP-H sequence is most similar to Genbank reference CAG33059.1 (human) and NCBI reference NP_001180163.1 (bos taurus).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET15b-hnRNP-H was a gift from Douglas Black (Addgene plasmid # 23020 ; http://n2t.net/addgene:23020 ; RRID:Addgene_23020) -
For your References section:
hnRNP H is a component of a splicing enhancer complex that activates a c-src alternative exon in neuronal cells. Chou MY, Rooke N, Turck CW, Black DL. Mol Cell Biol. 1999 Jan . 19(1):69-77. PubMed 9858532