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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 23019 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET15b
- Backbone size w/o insert (bp) 5700
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameKSRP
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SpeciesH. sapiens (human)
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Entrez GeneKHSRP (a.k.a. FBP2, FUBP2, KSRP, p75)
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Tag
/ Fusion Protein
- His (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET15b-KSRP was a gift from Douglas Black (Addgene plasmid # 23019 ; http://n2t.net/addgene:23019 ; RRID:Addgene_23019) -
For your References section:
A new regulatory protein, KSRP, mediates exon inclusion through an intronic splicing enhancer. Min H, Turck CW, Nikolic JM, Black DL. Genes Dev. 1997 Apr 15. 11(8):1023-36. 10.1101/gad.11.8.1023 PubMed 9136930
