pSEL2
(Plasmid
#23012)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 23012 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBAD/HisA
- Backbone size w/o insert (bp) 4100
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKanamycin resistance
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Speciesstandard selectable marker used in plasmids
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Insert Size (bp)870
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GenBank IDABP57185
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Tags
/ Fusion Proteins
- 6 His Tag (N terminal on backbone)
- Xpress epitope (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site HindIII (destroyed during cloning)
- 5′ sequencing primer pBAD forward
- 3′ sequencing primer pBAD reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSEL2 was a gift from James Bowie (Addgene plasmid # 23012 ; http://n2t.net/addgene:23012 ; RRID:Addgene_23012) -
For your References section:
Genetic selection system for improving recombinant membrane protein expression in E. coli. Massey-Gendel E, Zhao A, Boulting G, Kim HY, Balamotis MA, Seligman LM, Nakamoto RK, Bowie JU. Protein Sci. 2009 Feb . 18(2):372-83. 10.1002/pro.39 PubMed 19165721
Map uploaded by the depositor.
