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Addgene

pSEL1
(Plasmid #23011)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 23011 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD/HisA & pBThybrid
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DHFR
  • Alt name
    dihydrofolate reductase
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    620
  • GenBank ID
    NP_034179
  • Entrez Gene
    Dhfr (a.k.a. 8430436I03Rik)
  • Tags / Fusion Proteins
    • 6 His Tag (not in frame) (N terminal on backbone)
    • Xpress epitope (not in frame) (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site HindIII (destroyed during cloning)
  • 5′ sequencing primer pBAD forward
  • 3′ sequencing primer pBAD reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEL1 was a gift from James Bowie (Addgene plasmid # 23011 ; http://n2t.net/addgene:23011 ; RRID:Addgene_23011)

  • For your References section:

    Genetic selection system for improving recombinant membrane protein expression in E. coli. Massey-Gendel E, Zhao A, Boulting G, Kim HY, Balamotis MA, Seligman LM, Nakamoto RK, Bowie JU. Protein Sci. 2009 Feb . 18(2):372-83. 10.1002/pro.39 PubMed 19165721
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