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Addgene

pGFPCPB1
(Plasmid #23009)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 23009 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4731
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP-CenpB
  • Alt name
    CENP-B
  • Species
    Cricetulus griseus
  • Insert Size (bp)
    517
  • Mutation
    deleted CenpB amino acids 169-606, T145S
  • GenBank ID
    U20951
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGFPCPB1 was a gift from Linda Wordeman (Addgene plasmid # 23009 ; http://n2t.net/addgene:23009 ; RRID:Addgene_23009)

  • For your References section:

    MCAK facilitates chromosome movement by promoting kinetochore microtubule turnover. Wordeman L, Wagenbach M, von Dassow G. J Cell Biol. 2007 Dec 3. 179(5):869-79. 10.1083/jcb.200707120 PubMed 18039936
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