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Addgene

pLenti-HA-CBS(opt)-FLAG
(Plasmid #230088)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 230088 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 8220
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CBS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1696
  • Mutation
    Multiple wobble mutations
  • Entrez Gene
    CBS (a.k.a. CBSL, HIP4)
  • Promoter CMV
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • FLAG (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

codon-optomized, 5' Cloning Site: EcoRV (not destroyed), 3' Cloning Site: XhoI (not destroyed)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-HA-CBS(opt)-FLAG was a gift from Xingguo Zhu (Addgene plasmid # 230088 ; http://n2t.net/addgene:230088 ; RRID:Addgene_230088)

  • For your References section:

    Transsulfuration pathway activation attenuates oxidative stress and ferroptosis in sickle primary erythroblasts and transgenic mice. Xi C, Pang J, Xue W, Cui Y, Jiang N, Zhi W, Shi H, Horuzsko A, Pace BS, Zhu X. Commun Biol. 2025 Jan 6;8(1):15. doi: 10.1038/s42003-024-07424-7. 10.1038/s42003-024-07424-7 PubMed 39762627
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