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Addgene

pMX215
(Plasmid #23008)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 23008 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-M1
  • Backbone size w/o insert (bp) 4004
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAMFLhyp
  • Alt name
    MCAK
  • Alt name
    mitotic centromere-associated kinesin
  • Species
    Cricetulus griseus
  • Insert Size (bp)
    2943
  • GenBank ID
    U11790
  • Tag / Fusion Protein
    • mRFP1.0 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV promoter
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMX215 was a gift from Linda Wordeman (Addgene plasmid # 23008 ; http://n2t.net/addgene:23008 ; RRID:Addgene_23008)

  • For your References section:

    MCAK facilitates chromosome movement by promoting kinetochore microtubule turnover. Wordeman L, Wagenbach M, von Dassow G. J Cell Biol. 2007 Dec 3. 179(5):869-79. 10.1083/jcb.200707120 PubMed 18039936
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