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Addgene

pMX234
(Plasmid #23006)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 23006 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-M1
  • Backbone size w/o insert (bp) 4004
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mRFP-CenpB
  • Alt name
    CENP-B
  • Alt name
    centromere autoantigen protein B
  • Species
    Cricetulus griseus
  • Insert Size (bp)
    1214
  • Mutation
    deleted CenpB amino acids 169-606, T145S
  • GenBank ID
    U20951
  • Tag / Fusion Protein
    • mRFP1.0 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV promoter
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMX234 was a gift from Linda Wordeman (Addgene plasmid # 23006 ; http://n2t.net/addgene:23006 ; RRID:Addgene_23006)

  • For your References section:

    The kinesin-8 motor Kif18A suppresses kinetochore movements to control mitotic chromosome alignment. Stumpff J, von Dassow G, Wagenbach M, Asbury C, Wordeman L. Dev Cell. 2008 Feb . 14(2):252-62. 10.1016/j.devcel.2007.11.014 PubMed 18267093
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