pAAV-hSyn-GluA1-scFv-Halo
(Plasmid
#230055)
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PurposeAAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 fused to halotag. Can be directly transfected into neurons or used to prepare AAV expressing GluA1-scFv-Halo.
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 230055 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAAV hSyn
- Backbone size w/o insert (bp) 4496
- Total vector size (bp) 6265
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAnti-GluA1 single chain variable fragment (scFv)
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SpeciesM. musculus (mouse)
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Insert Size (bp)1726
- Promoter human synapsin
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Tag
/ Fusion Protein
- HaloTag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer cactgccagcttcagca (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hSyn-GluA1-scFv-Halo was a gift from Matthew Kennedy (Addgene plasmid # 230055 ; http://n2t.net/addgene:230055 ; RRID:Addgene_230055)
