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Addgene

TB204 △ilvA
(Bacterial strain #230041)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 230041 Bacteria in agar stab 1 $85
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Backbone

  • Vector backbone
    NA
  • Selectable markers
    sfGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    None
  • Growth Temperature
    37°C
  • Growth Strain(s)
    TB204
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MG1655 attP21::PR-sfGFP::frt ilvA::frt

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Strain Validation: Fluorescence, growth in M9+glucose +/- isoleucine, PCRs with primers flanking fluorescent marker gene or deleted gene.
Primers:
ilvA_fwd: ATCGTGAACGTCAGGTCTCC
ilvA_rev: TCTGGAAGATTTTGCCGAAC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TB204 △ilvA was a gift from Martin Ackermann & Olga Schubert (Addgene plasmid # 230041)

  • For your References section:

    Minorities drive growth resumption in cross-feeding microbial communities. Micali G, Hockenberry AM, Dal Co A, Ackermann M. Proc Natl Acad Sci U S A. 2023 Nov 7;120(45):e2301398120. doi: 10.1073/pnas.2301398120. Epub 2023 Oct 30. 10.1073/pnas.2301398120 PubMed 37903278
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