Skip to main content
Addgene

TB204 △metA
(Bacterial strain #230039)

Print

Full plasmid sequence is not available for this item.

No maps are available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 230039 Bacteria in agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    NA
  • Selectable markers
    sfGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    None
  • Growth Temperature
    37°C
  • Growth Strain(s)
    TB204
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MG1655 attP21::PR-sfGFP::frt metA::frt

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Strain Validation: Fluorescence, growth in M9+glucose +/- methionine, PCRs with primers flanking fluorescent marker gene or deleted gene.
Primers:
metA_fwd: CGTGAGCGGCGAATACTAAT
metA_rev: AAACTTCCCCACTGTGAAC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TB204 △metA was a gift from Martin Ackermann & Olga Schubert (Addgene plasmid # 230039)

  • For your References section:

    Minorities drive growth resumption in cross-feeding microbial communities. Micali G, Hockenberry AM, Dal Co A, Ackermann M. Proc Natl Acad Sci U S A. 2023 Nov 7;120(45):e2301398120. doi: 10.1073/pnas.2301398120. Epub 2023 Oct 30. 10.1073/pnas.2301398120 PubMed 37903278
Related items:
Commonly requested with: