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Addgene

TB204 △trpC
(Bacterial strain #230037)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 230037 Bacteria in agar stab 1 $85
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Backbone

  • Vector backbone
    NA
  • Selectable markers
    sfGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    None
  • Growth Temperature
    37°C
  • Growth Strain(s)
    TB204
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MG1655 attP21::PR-sfGFP::frt trpC::frt

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Strain Validation: Fluorescence, growth in M9+glucose +/- tryptophane, PCRs with primers flanking fluorescent marker gene or deleted gene.
Primers:
trpC_fwd: AACGTCGCCATGTTAATGCG
trpC_rev: GAACTGAGCCTGAAATTCAGG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TB204 △trpC was a gift from Martin Ackermann & Olga Schubert (Addgene plasmid # 230037)

  • For your References section:

    Short-range interactions govern the dynamics and functions of microbial communities. Dal Co A, van Vliet S, Kiviet DJ, Schlegel S, Ackermann M. Nat Ecol Evol. 2020 Mar;4(3):366-375. doi: 10.1038/s41559-019-1080-2. Epub 2020 Feb 10. 10.1038/s41559-019-1080-2 PubMed 32042125
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