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Addgene

TB204
(Bacterial strain #230033)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 230033 Bacteria in agar stab 1 $85
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Backbone

  • Vector backbone
    NA
  • Selectable markers
    sfGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    None
  • Growth Temperature
    37°C
  • Growth Strain(s)
    TB204
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MG1655 attP21::PR-sfGFP::frt

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Strain Validation: Fluorescence, PCR for fluorescent marker gene.
Inserted into attP21 and PCR-verified following methods described in Haldimann and Wanner, JBact 2001, PMID 11591683, using pAH68FRT-Cat as cloning and insertion plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TB204 was a gift from Tobias Bergmiller & Calin Guet (Addgene plasmid # 230033)

  • For your References section:

    Lack of cations in flow cytometry buffers affect fluorescence signals by reducing membrane stability and viability of Escherichia coli strains. Tomasek K, Bergmiller T, Guet CC. J Biotechnol. 2018 Feb 20;268:40-52. doi: 10.1016/j.jbiotec.2018.01.008. Epub 2018 Jan 20. 10.1016/j.jbiotec.2018.01.008 PubMed 29355812
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