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Addgene

pMX229
(Plasmid #23002)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 23002 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4731
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kif18A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2731
  • GenBank ID
    BC048347
  • Entrez Gene
    KIF18A (a.k.a. MS-KIF18A, PPP1R99)
  • Tags / Fusion Proteins
    • EGFP (N terminal on backbone)
    • 6His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMX229 was a gift from Linda Wordeman (Addgene plasmid # 23002 ; http://n2t.net/addgene:23002 ; RRID:Addgene_23002)

  • For your References section:

    The kinesin-8 motor Kif18A suppresses kinetochore movements to control mitotic chromosome alignment. Stumpff J, von Dassow G, Wagenbach M, Asbury C, Wordeman L. Dev Cell. 2008 Feb . 14(2):252-62. 10.1016/j.devcel.2007.11.014 PubMed 18267093
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