pEGFPC1-6XHis-FLKSRP
(Plasmid #23001)

• Depositing Lab: Douglas Black
• Publication: Hall et al Mol Biol Cell. 2004 Feb . 15(2):774-86.

Backbone

• Vector backbone: pEGFP-C1
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KSRP
• Species: H. sapiens (human)
• Mutation: valine inserted at aa#2
• Entrez Gene: KHSRP (a.k.a. FBP2, FUBP2, KSRP, p75)
• Tags / Fusion Proteins:
  • EGFP (N terminal on insert)
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: EGFP-C

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The KHSRP insert in this plasmid contains an extra amino acid, valine, at position #2.