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pCM100_ PB5'IR-hU6-gRNA-CS1- CAG-tdTomato- PB3'IR
(Plasmid #229995)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229995 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Piggybac
  • Backbone size (bp) 3000
  • Vector type
    Mammalian Expression, CRISPR
  • Promoter CAG
  • Tag / Fusion Protein
    • NotI site for NEBuilder HiFi DNA Assembly with ssDNA oligo cttgtggaaaggacgaaaCACCG(G)-sgRNA-gtttaagagctatgctggaa

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCM100_ PB5'IR-hU6-gRNA-CS1- CAG-tdTomato- PB3'IR was a gift from Christian Mayer (Addgene plasmid # 229995 ; http://n2t.net/addgene:229995 ; RRID:Addgene_229995)

  • For your References section:

    Spatial enhancer activation influences inhibitory neuron identity during mouse embryonic development. Dvoretskova E, Ho MC, Kittke V, Neuhaus F, Vitali I, Lam DD, Delgado I, Feng C, Torres M, Winkelmann J, Mayer C. Nat Neurosci. 2024 May;27(5):862-872. doi: 10.1038/s41593-024-01611-9. Epub 2024 Mar 25. 10.1038/s41593-024-01611-9 PubMed 38528203
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