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Addgene

pPLP2S-mCf
(Plasmid #229986)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229986 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEN6
  • Backbone manufacturer
    In-house
  • Backbone size (bp) 2177
  • Modifications to backbone
    Contains expression cassette for (modified) MS2 capsid proteins and control RNA with pac sequences. A non-coding fragment of mCherry is cloned into the control LIC site.
  • Vector type
    Bacterial Expression
  • Promoter T7
  • Tag / Fusion Protein
    • Strep-tag II

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPLP2S-mCf was a gift from Barry Lutz (Addgene plasmid # 229986 ; http://n2t.net/addgene:229986 ; RRID:Addgene_229986)

  • For your References section:

    Simplified MS2 phage-like particle production including a novel maturation protein internal fusion affinity tag. Kline EC, Duong R, Wang Q, & Lutz BR.. Biotechnol Biotechnol Equip. 2025 Feb;39(1). doi: 10.1080/13102818.2025.2457691. Epub 2025 Feb 2. 10.1080/13102818.2025.2457691
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