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Addgene

pcDNA FLAG mTOR wt Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: BamHI and KpnI (both HF versions).

We expected the following band sizes for this digest: 5.9kb, 5.4kb, and 1.7kb.. We see the expected pattern.