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Addgene

pDONOR 6colors
(Plasmid #229974)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229974 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBIG
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mito-mCherry, EYFP-tubulin, H2B iRFP720, GTS-mTagBFP, mTFP1-actin
  • Species
    H. sapiens (human), Synthetic
  • Mutation
    See Depositor Comments

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's NGS results found F394L mutation in EYFP-tubulin that does not affect plasmid function.

Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONOR 6colors was a gift from Martin Pelosse (Addgene plasmid # 229974 ; http://n2t.net/addgene:229974 ; RRID:Addgene_229974)

  • For your References section:

    biGMamAct: efficient CRISPR/Cas9-mediated docking of large functional DNA cargoes at the ACTB locus. Pelosse M, Marcia M. Synthetic Biology 2025.02.13 10.1093/synbio/ysaf003
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