pET29-neoLux1.2
(Plasmid
#229952)
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PurposeBacterial expression for de novo luciferase, neoLux1.2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 229952 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET29
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5214
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameneoLux1.2
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SpeciesSynthetic
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Insert Size (bp)402
- Promoter T7
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Tag
/ Fusion Protein
- His-tag (C terminal on insert)
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET29-neoLux1.2 was a gift from Andy Yeh (Addgene plasmid # 229952 ; http://n2t.net/addgene:229952 ; RRID:Addgene_229952) -
For your References section:
De novo luciferases enable multiplexed bioluminescence imaging. Chen JY, Shi Q, Peng X, De Dieu Habimana J, Wang J, Sobolewski W, Yeh AH. Chem. 2024 Nov 12. doi: 10.1016/j.chempr.2024.10.013 10.1016/j.chempr.2024.10.013