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Addgene

Mad GSK3
(Plasmid #22995)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22995 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4096
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mad
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1300
  • Mutation
    S208A, S204A
  • Entrez Gene
    Mad (a.k.a. Dmel_CG12399, 2/23, CG12399, Dmel\CG12399, E(zen)2, En(vvl), MAD, Mat, P-Mad, P-mad, PMad, Smad, Smad1, apg, c28, dMAD, dMad, l(2)K00237, l(2)k00237, mad, p-MAD, p-Mad, pMAD, pMad, pSmad)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mad GSK3 was a gift from Edward De Robertis (Addgene plasmid # 22995 ; http://n2t.net/addgene:22995 ; RRID:Addgene_22995)

  • For your References section:

    Mad is required for wingless signaling in wing development and segment patterning in Drosophila. Eivers E, Fuentealba LC, Sander V, Clemens JC, Hartnett L, De Robertis EM. PLoS One. 2009 . 4(8):e6543. 10.1371/journal.pone.0006543 PubMed 19657393
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