pcDNA3-luxNeon
(Plasmid
#229947)
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PurposeMammalian expression of a de novo designed, FRET-based neoLux1.2-mNeonGreen fusion bioluminescent probe (max. Em=518nm)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 229947 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5372
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameluxNeon
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SpeciesSynthetic
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Insert Size (bp)1050
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-luxNeon was a gift from Andy Yeh (Addgene plasmid # 229947 ; http://n2t.net/addgene:229947 ; RRID:Addgene_229947) -
For your References section:
De novo luciferases enable multiplexed bioluminescence imaging. Chen JY, Shi Q, Peng X, De Dieu Habimana J, Wang J, Sobolewski W, Yeh AH. Chem. 2024 Nov 12. doi: 10.1016/j.chempr.2024.10.013 10.1016/j.chempr.2024.10.013
