pBAD-luxKOk
(Plasmid
#229944)
-
PurposeBacterial expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 229944 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBAD
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3955
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameluxKOk
-
SpeciesSynthetic
-
Insert Size (bp)1044
- Promoter araBAD
-
Tag
/ Fusion Protein
- His-tag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pBAD Forward
- 3′ sequencing primer pBAD Reverse (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBAD-luxKOk was a gift from Andy Yeh (Addgene plasmid # 229944 ; http://n2t.net/addgene:229944 ; RRID:Addgene_229944) -
For your References section:
De novo luciferases enable multiplexed bioluminescence imaging. Chen JY, Shi Q, Peng X, De Dieu Habimana J, Wang J, Sobolewski W, Yeh AH. Chem. 2024 Nov 12. doi: 10.1016/j.chempr.2024.10.013 10.1016/j.chempr.2024.10.013