pCS2 hSmad1-EVE-MM
(Plasmid
#22994)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22994 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepCS2
- Backbone size w/o insert (bp) 4096
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameSmad1
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1300
-
MutationS214A, S206A, S195A, S187A, S463E, S465E
-
Entrez GeneSMAD1 (a.k.a. BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1)
-
Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCS2 hSmad1-EVE-MM was a gift from Edward De Robertis (Addgene plasmid # 22994 ; http://n2t.net/addgene:22994 ; RRID:Addgene_22994) -
For your References section:
Integrating patterning signals: Wnt/GSK3 regulates the duration of the BMP/Smad1 signal. Fuentealba LC, Eivers E, Ikeda A, Hurtado C, Kuroda H, Pera EM, De Robertis EM. Cell. 2007 Nov 30. 131(5):980-93. 10.1016/j.cell.2007.09.027 PubMed 18045539