pHW1387
(Plasmid
#229880)
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PurposeNegative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 229880 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMLS257
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Backbone manufacturerErik Jorgensen, Addgene plasmid #73716
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
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SpeciesC. elegans (nematode), Synthetic
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Tag
/ Fusion Protein
- GFP-C1 (C terminal on insert)
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHW1387 was a gift from Han Wang (Addgene plasmid # 229880 ; http://n2t.net/addgene:229880 ; RRID:Addgene_229880) -
For your References section:
A versatile site-directed gene trap strategy to manipulate gene activity and control gene expression in Caenorhabditis elegans. Khan H, Huang X, Raj V, Wang H. PLoS Genet. 2025 Jan 22;21(1):e1011541. doi: 10.1371/journal.pgen.1011541. eCollection 2025 Jan. 10.1371/journal.pgen.1011541 PubMed 39841730