pHW1133
(Plasmid
#229869)
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Purpose(Empty Backbone) Destination RMCE vector containing FRT::SA::51-bp Artificial exon::3xStop::SL2::SapI Insertion sites (to clone new driver with 3'UTR in slot 5 and SEC in slot 6)::reverse FRT3 to swap driver via RMCE
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 229869 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMLS257
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Backbone manufacturerErik Jorgensen, Addgene plasmid #73716
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHW1133 was a gift from Han Wang (Addgene plasmid # 229869 ; http://n2t.net/addgene:229869 ; RRID:Addgene_229869) -
For your References section:
A versatile site-directed gene trap strategy to manipulate gene activity and control gene expression in Caenorhabditis elegans. Khan H, Huang X, Raj V, Wang H. PLoS Genet. 2025 Jan 22;21(1):e1011541. doi: 10.1371/journal.pgen.1011541. eCollection 2025 Jan. 10.1371/journal.pgen.1011541 PubMed 39841730
