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Addgene

pHW622
(Plasmid #229868)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229868 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNM3456
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    reverse FRT3 (rFRT3)
  • Species
    C. elegans (nematode), Synthetic

Cloning Information

  • Cloning method Golden Gate

Resource Information

  • Supplemental Documents

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHW622 was a gift from Han Wang (Addgene plasmid # 229868 ; http://n2t.net/addgene:229868 ; RRID:Addgene_229868)

  • For your References section:

    A versatile site-directed gene trap strategy to manipulate gene activity and control gene expression in Caenorhabditis elegans. Khan H, Huang X, Raj V, Wang H. PLoS Genet. 2025 Jan 22;21(1):e1011541. doi: 10.1371/journal.pgen.1011541. eCollection 2025 Jan. 10.1371/journal.pgen.1011541 PubMed 39841730
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