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pFastBac-Dual_FluA/H7N9
(Plasmid #229860)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229860 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac-Dual
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Gentamicin, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    PA_H7N9-FluA
  • GenBank ID
    AGJ51952.1
  • Tag / Fusion Protein
    • 8xHis-tag (N terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    PB1_H7N9-FluA
  • GenBank ID
    AGJ51960.1

Gene/Insert 3

  • Gene/Insert name
    PB2_H7N9-FluA
  • GenBank ID
    KJ633805.1
  • Tag / Fusion Protein
    • 2x-Streptag (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac-Dual_FluA/H7N9 was a gift from Stephen Cusack (Addgene plasmid # 229860 ; http://n2t.net/addgene:229860 ; RRID:Addgene_229860)

  • For your References section:

    Direct observation of backtracking by influenza A and B polymerases upon consecutive incorporation of the nucleoside analog T1106. Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S. Cell Rep. 2023 Jan 31;42(1):111901. doi: 10.1016/j.celrep.2022.111901. Epub 2023 Jan 2. 10.1016/j.celrep.2022.111901 PubMed 36596301
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