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pKLBac-FluB-trimer
(Plasmid #229859)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229859 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKLPBac
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin and Gentamicin, 50 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    PA, Influenza B (B/Memphis/13/03)
  • GenBank ID
  • Tag / Fusion Protein
    • 8xHis-tag (N terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    PB1, Influenza B (B/Memphis/13/03)
  • GenBank ID

Gene/Insert 3

  • Gene/Insert name
    PB2, Influenza B (B/Los Angeles/1/02)
  • GenBank ID
  • Tag / Fusion Protein
    • 2x-Streptag (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKLBac-FluB-trimer was a gift from Stephen Cusack (Addgene plasmid # 229859 ; http://n2t.net/addgene:229859 ; RRID:Addgene_229859)

  • For your References section:

    Structural insight into cap-snatching and RNA synthesis by influenza polymerase. Reich S, Guilligay D, Pflug A, Malet H, Berger I, Crepin T, Hart D, Lunardi T, Nanao M, Ruigrok RW, Cusack S. Nature. 2014 Dec 18;516(7531):361-6. doi: 10.1038/nature14009. Epub 2014 Nov 19. 10.1038/nature14009 PubMed 25409151
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