pKLBac-Bat-FluA-trimer
(Plasmid
#229858)
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PurposeInfluenza A/little yellow-shouldered bat/Guatemala/060/2010(H17N10) polymerase heterotrimer (self-cleaving polyprotein)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 229858 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepKLPBac
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin and Gentamicin, 50 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namePA_Bat-FluA_AFC35437.1
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GenBank IDAFC35437.1
Gene/Insert 2
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Gene/Insert namePB1_Bat-FluA_AFC35436
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GenBank IDAFC35436
Gene/Insert 3
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Gene/Insert namePB2_Bat-FluA_AFC35435.1
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GenBank IDAFC35435.1
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKLBac-Bat-FluA-trimer was a gift from Stephen Cusack (Addgene plasmid # 229858 ; http://n2t.net/addgene:229858 ; RRID:Addgene_229858) -
For your References section:
Structure of influenza A polymerase bound to the viral RNA promoter. Pflug A, Guilligay D, Reich S, Cusack S. Nature. 2014 Dec 18;516(7531):355-60. doi: 10.1038/nature14008. Epub 2014 Nov 19. 10.1038/nature14008 PubMed 25409142