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pSG5LHARB1sgresistant
(Plasmid #229856)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229856 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSG5L
  • Backbone manufacturer
    4000
  • Backbone size w/o insert (bp) 2800
  • Modifications to backbone
    The RB1 was originally cloned by Dr. William Sellers (Plasmid #10720).
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    RB1
  • Species
    H. sapiens (human)
  • Mutation
    changed a bp24 to g; g bp27 to c, and c bp30 to g. These are silenced mutations.
  • Entrez Gene
    RB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Rb1
  • Alt name
    OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110
  • Species
    H. sapiens (human)
  • Entrez Gene
    RB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    William Sellers originally cloned this gene (Plasmid #10720).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG5LHARB1sgresistant was a gift from Maria Alvarado-Kristensson (Addgene plasmid # 229856 ; http://n2t.net/addgene:229856 ; RRID:Addgene_229856)

  • For your References section:

    Targeting TUBG1 in RB1-negative tumors. Lindstrom L, Zhou J, Villoutreix BO, Malycheva D, Otrocka M, Gustavsson AL, Lundback T, Bliman D, Shameem MA, Straw M, Riesbeck K, Olsson R, Alvarado-Kristensson M. FASEB J. 2025 Mar 15;39(5):e70431. doi: 10.1096/fj.202403180RR. 10.1096/fj.202403180RR PubMed 40019206
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