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Addgene

pBS-ACTB-C200-T7
(Plasmid #229850)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229850 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBlueScript II SK (+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2959
  • Total vector size (bp) 3184
  • Modifications to backbone
    None
  • Vector type
    HR donor vector for human ACTB gene.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Partial sequence of ACTB gene
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    401

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Small InDels may occur in the regions with consecutive thymine (T) sequences; single nucleotide insertion in the ACTB partial gene sequence is likely not a concern for plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-ACTB-C200-T7 was a gift from Natsuko Chiba (Addgene plasmid # 229850 ; http://n2t.net/addgene:229850 ; RRID:Addgene_229850)
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