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Addgene

pCG-NT2
(Plasmid #229848)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229848 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LentiCRISPRv2
  • Backbone manufacturer
    Addgene Plasmid #52961
  • Backbone size w/o insert (bp) 12993
  • Total vector size (bp) 6937
  • Modifications to backbone
    Remove sequences required for lentiviral packaging and puromycin selection.
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    guide RNA with non-target sequence
  • gRNA/shRNA sequence
    GTTCAGGATCACGTTACCGC
  • Species
    H. sapiens (human)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCG-NT2 was a gift from Natsuko Chiba (Addgene plasmid # 229848 ; http://n2t.net/addgene:229848 ; RRID:Addgene_229848)
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