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Addgene

T7-p73DD-pcDNA3
(Plasmid #22978)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22978 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5484
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tumor protein p73
  • Alt name
    TP73
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    942
  • Mutation
    truncated cDNA encodes truncated protein (aa 327-636) with dominant negative function
  • GenBank ID
    NM_005427
  • Entrez Gene
    TP73 (a.k.a. CILD47, P73)
  • Tag / Fusion Protein
    • T7 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (not destroyed)
  • 3′ cloning site EcoR I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T7-p73DD-pcDNA3 was a gift from William Kaelin (Addgene plasmid # 22978 ; http://n2t.net/addgene:22978 ; RRID:Addgene_22978)

  • For your References section:

    Role for the p53 homologue p73 in E2F-1-induced apoptosis. Irwin M, Marin MC, Phillips AC, Seelan RS, Smith DI, Liu W, Flores ER, Tsai KY, Jacks T, Vousden KH, Kaelin WG. Nature. 2000 Oct 5. 407(6804):645-8. 10.1038/35036614 PubMed 11034215
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