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Addgene

AiP12068 - pAAV-DLX2.0-minBG-ChrimsonR-tdTomato-WPRE3-BGHpA (Alias: CN2068)
(Plasmid #229775)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229775 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ChrimsonR-tdTomato
  • Species
    Synthetic
  • Insert Size (bp)
    2502
  • Tag / Fusion Protein
    • tdTomato (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Edward Boyden (Addgene plasmid # 59171); the ChrimsonR-tdTomato was subcloned from it.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.11.01.621418 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AiP12068 - pAAV-DLX2.0-minBG-ChrimsonR-tdTomato-WPRE3-BGHpA (Alias: CN2068) was a gift from The Allen Institute for Brain Science & Jonathan Ting (Addgene plasmid # 229775 ; http://n2t.net/addgene:229775 ; RRID:Addgene_229775)

  • For your References section:

    Simultaneous mesoscopic measurement and manipulation of mouse cortical activity. Matveev P, Li AJ, Ye Z, Bowen AJ, Opitz-Araya X, Ting JT, Steinmetz NA. bioRxiv 2024.11.01.621418 10.1101/2024.11.01.621418
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