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pFN35K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)
(Plasmid #229767)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229767 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFN35K SmBiT TK-neo Flexi vector
  • Backbone manufacturer
    Promega
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human collagen type IV alpha 3 chain (COL4A3)
  • Species
    H. sapiens (human)
  • Mutation
    See Depositor Comments
  • Entrez Gene
    COL4A3 (a.k.a. ATS2, ATS3, ATS3A, ATS3B, BFH2)
  • Tag / Fusion Protein
    • SmBiT (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFN35K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3) was a gift from Hirofumi Kai (Addgene plasmid # 229767 ; http://n2t.net/addgene:229767 ; RRID:Addgene_229767)

  • For your References section:

    A Split-Luciferase-Based Trimer Formation Assay as a High-throughput Screening Platform for Therapeutics in Alport Syndrome. Omachi K, Kamura M, Teramoto K, Kojima H, Yokota T, Kaseda S, Kuwazuru J, Fukuda R, Koyama K, Matsuyama S, Motomura K, Shuto T, Suico MA, Kai H. Cell Chem Biol. 2018 May 17;25(5):634-643.e4. doi: 10.1016/j.chembiol.2018.02.003. Epub 2018 Mar 8. 10.1016/j.chembiol.2018.02.003 PubMed 29526710
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