pcDNA FLAG TORC1 (1-630)
(Plasmid #22974)

• Depositing Lab: Marc Montminy
• Publication: Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

Backbone

• Vector backbone: pcDNA3.0
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TORC1 (1-630)
• Alt name: TORC1
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Mutation: Amino acids 1-630 rather than 1-634.
• Entrez Gene: CRTC1 (a.k.a. MAML2, MECT1, Mam-2, TORC-1, TORC1, WAMTP1)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is an XbaI site at the 3' end instead of a NotI site.